Journal: Journal of the Endocrine Society

Article Title: 17 β -Estradiol Promotes Islet Cell Proliferation in a Partial Pancreatectomy Mouse Model

doi: 10.1210/js.2016-1073

Figure Lengend Snippet: Antibody Table

Article Snippet: FOXM1 , C-20 , FoxM1 , Santa Cruz Biotechnology, sc-502 , Rabbit, polyclonal , 1:200 , RRID:AB_631523.

Techniques:

Journal: Journal of the Endocrine Society

Article Title: 17 β -Estradiol Promotes Islet Cell Proliferation in a Partial Pancreatectomy Mouse Model

doi: 10.1210/js.2016-1073

Figure Lengend Snippet: Effect of E2 treatment on the cell cycle regulators. (a) Two weeks after the PPx surgery, the mice were killed, and the isolated islets were processed for immunoblotting analysis of FOXM1, cyclin A2, cyclin B1, cyclin B2, cyclin D2, and cyclin E2. (b) The protein expression was quantified and normalized to the expression of α -tubulin. Each data point was the mean ± SEM of three independent experiments. * P < 0.05, ** P < 0.01.

Article Snippet: FOXM1 , C-20 , FoxM1 , Santa Cruz Biotechnology, sc-502 , Rabbit, polyclonal , 1:200 , RRID:AB_631523.

Techniques: Isolation, Western Blot, Expressing

Journal: Cell Death Discovery

Article Title: Small molecule targeting FOXM1 DNA binding domain exhibits anti-tumor activity in ovarian cancer

doi: 10.1038/s41420-022-01070-w

Figure Lengend Snippet: A , B The inhibitor effect of 15 candidate compounds on ovarian cancer cell lines were measured by MTT assay. C , D Expression level of CCNB1 was detected by qPCR in A2780 cells treated with compounds. E – H Inhibitor effect of XST-20 on four ovarian cancer cell lines was evaluated by MTT assay. Data were presented as means ± S.D. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: The primary antibodies were as follows: rabbit anti-p21Waf1/Cip1 (12D1) monoclonal antibody (8831, Cell Signaling Technology,1:1000); rabbit anti-CCNB1 polyclonal antibody (D160234,Sangon Biotech,1:500); mouse anti-cyclinD1(A-12) monoclonal antibody (sc-8396, Santa Cruz Biotechnology, 1:200); mouse anti-p27 Kip1 (F-8) monoclonal antibody (sc-1641, Santa Cruz Biotechnology, 1:200); mouse anti-Plk (F-8) monoclonal antibody (sc-17783, Santa Cruz Biotechnology,1:100); mouse anti-FOXM1(G-5) monoclonal antibody (sc-376471,Santa Cruz Biotechnology,1:100); mouse anti-β-actin monoclonal antibody (66009-l-lg,Proteintech, 1:8000); mouse anti-β-Tubulin (C66) monoclonal antibody (M20005S, Abmart, 1:5000).

Techniques: MTT Assay, Expressing

Journal: Cell Death Discovery

Article Title: Small molecule targeting FOXM1 DNA binding domain exhibits anti-tumor activity in ovarian cancer

doi: 10.1038/s41420-022-01070-w

Figure Lengend Snippet: A Interaction between XST-20 and FOXM1 (222–360) peptide was detected by surface plasmon resonance (SPR) assay. B Luciferase activity of CCNB1 promoter was evaluated in cells with FOXM1 overexpression. C Luciferase activity of CCNB1 promoter was evaluated in cells with FOXM1 overexpression and control group treated with XST-20. D Expression levels of FOXM1 and downstream targets were Measured by western blot in three ovarian cancer cell lines (A2780, HEY, and SKOV3) treated with XST-20. Data were presented as means ± S.D. * p < 0.05, ** p < 0.01.

Article Snippet: The primary antibodies were as follows: rabbit anti-p21Waf1/Cip1 (12D1) monoclonal antibody (8831, Cell Signaling Technology,1:1000); rabbit anti-CCNB1 polyclonal antibody (D160234,Sangon Biotech,1:500); mouse anti-cyclinD1(A-12) monoclonal antibody (sc-8396, Santa Cruz Biotechnology, 1:200); mouse anti-p27 Kip1 (F-8) monoclonal antibody (sc-1641, Santa Cruz Biotechnology, 1:200); mouse anti-Plk (F-8) monoclonal antibody (sc-17783, Santa Cruz Biotechnology,1:100); mouse anti-FOXM1(G-5) monoclonal antibody (sc-376471,Santa Cruz Biotechnology,1:100); mouse anti-β-actin monoclonal antibody (66009-l-lg,Proteintech, 1:8000); mouse anti-β-Tubulin (C66) monoclonal antibody (M20005S, Abmart, 1:5000).

Techniques: SPR Assay, Luciferase, Activity Assay, Over Expression, Expressing, Western Blot